5ZRR: Crystal structure of PET-degrading cutinase Cut190 S176A/S226P/R228S mutant in monoethyl succinate bound state
X-ray · 1.34 Å · released 12 Sep 2018 · Saccharomonospora viridis
Related entries for the same target
same UniProt accessionLigand: 9J3
How it was solved
- Method
- X-ray
- Resolution
- 1.34 Å
- Released
- 12 Sep 2018
- Rfree
- 0.185
- Rwork
- 0.162
- Clashscore
- 0.49
- Ramachandran outliers
- 0.00%
- Rotamer outliers
- 0.00%
Crystallisation
- Technique
- Vapor Diffusion, Hanging Drop
- pH
- 6.5
- Temperature
- 293.0 K
0.01 M ZnSO4, 0.1 M MES pH 6.5, 25% v/v PEG MME 550
What it led to
PANTS: Mines metagenomes for uncharacterised PETase and MHETase candidates, ranked by catalytic triad and oxyanion hole
Playbook · 15 Sep 2026
Do it yourself
Field Guide: Xray and crystallisation
Unlocking Membrane Protein Structures: The Crystallization Revolution Reshaping Drug Discovery
Engineering Protein Stability: Unlocking the Secrets of Successful Crystallization
AXIS: Closing the Loop on Crystal Detection
Playbook
The Self-Driving Lab: How Automation and AI Are Redefining the Pace of Discovery
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