4WFK: Crystal structure of PET-degrading cutinase Cut190 S226P mutant in Ca(2+)-bound state at 2.35 angstrom resolution
X-ray · 2.35 Å · released 24 Dec 2014 · Saccharomonospora viridis
Related entries for the same target
same UniProt accessionHow it was solved
- Method
- X-ray
- Resolution
- 2.35 Å
- Released
- 24 Dec 2014
- Rfree
- 0.224
- Rwork
- 0.168
- Clashscore
- 2.02
- Ramachandran outliers
- 0.39%
- Rotamer outliers
- 0.47%
Crystallisation
- Technique
- Vapor Diffusion, Sitting Drop
- pH
- 7.0
- Temperature
- 293.0 K
0.2 M sodium fluoride, 20% (w/v) PEG 3350
What it led to
PANTS: Mines metagenomes for uncharacterised PETase and MHETase candidates, ranked by catalytic triad and oxyanion hole
Playbook · 15 Sep 2026
Do it yourself
Field Guide: Xray and crystallisation
Unlocking Membrane Protein Structures: The Crystallization Revolution Reshaping Drug Discovery
Engineering Protein Stability: Unlocking the Secrets of Successful Crystallization
AXIS: Closing the Loop on Crystal Detection
Playbook
The Self-Driving Lab: How Automation and AI Are Redefining the Pace of Discovery
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