5ZNO: Crystal structure of PET-degrading cutinase Cut190 S176A/S226P/R228S/ mutant in Ca(2+)-bound state
X-ray · 1.60 Å · released 12 Sep 2018 · Saccharomonospora viridis
Related entries for the same target
same UniProt accessionHow it was solved
- Method
- X-ray
- Resolution
- 1.60 Å
- Released
- 12 Sep 2018
- Rfree
- 0.222
- Rwork
- 0.179
- Clashscore
- 1.49
- Ramachandran outliers
- 0.00%
- Rotamer outliers
- 0.46%
Crystallisation
- Technique
- Vapor Diffusion, Hanging Drop
- pH
- 7.5
- Temperature
- 293.0 K
0.1 M HEPES pH 7.5, 10% w/v PEG 8000, 8% v/v Ethylene glycol
What it led to
PANTS: Mines metagenomes for uncharacterised PETase and MHETase candidates, ranked by catalytic triad and oxyanion hole
Playbook · 15 Sep 2026
Do it yourself
Field Guide: Xray and crystallisation
Unlocking Membrane Protein Structures: The Crystallization Revolution Reshaping Drug Discovery
Engineering Protein Stability: Unlocking the Secrets of Successful Crystallization
AXIS: Closing the Loop on Crystal Detection
Playbook
The Self-Driving Lab: How Automation and AI Are Redefining the Pace of Discovery
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