8H5K: Crystal structure of PETase N37D/S121E/R132E/A171C/A180V/P181V/D186H/S193C/R224E/N233C/S242T/N246D/S282C mutant from Ideonella sakaiensis

Crystallisation

Technique

Vapor Diffusion, Sitting Drop

Temperature

295.0 K

15 % PEG3350, 0.1 M Sodium HEPES pH7.0, 0.01 M Magnesium chloride hexahydrate, and 0.005 M Nickel (II) chloride hexahydrate

Primary citation

A case of balance engineering exhibits kinetic relationship between mesophilic and thermophilic poly(ethylene terephthalate) depolymerases.
To Be Published None

Tools

GOBSMACKED ↗
fold, dock, relax and annotate a protein-ligand complex on your own GPU, then find the crystal structure and g

CODSWALLOP ↗
every PDB entry for a protein family in one place: what was actually made, what it bound, what crystallised, a

TopPDBLX ↗
every crystallisation condition in the protein data bank, parsed and indexed: what worked, how often, and how

FlexAppeal ↗
Set up protein molecular dynamics without writing the script: every OpenMM option explained, a self-contained

BoltzMaker ↗
Build and analyze Boltz-2 protein structure prediction campaigns: a guided input wizard, YAML generation, and