7QVH: The crystal structure of HotPETase, an evolved thermostable variant of IsPETase
X-ray · 2.24 Å · released 3 Aug 2022 · Pseudideonella sakaiensis
How it was solved
- Method
- X-ray
- Resolution
- 2.24 Å
- Released
- 3 Aug 2022
- Rfree
- 0.251
- Rwork
- 0.198
- Clashscore
- 1.03
- Ramachandran outliers
- 0.00%
- Rotamer outliers
- 0.00%
Crystallisation
- Technique
- Vapor Diffusion, Sitting Drop
- Temperature
- 293.0 K
0.85 M sodium citrate tribasic dehydrate, 0.1 M Tris pH 8.0 and 0.1 M sodium chloride (LMB screen HT96 H7 Molecular Dimensions).
What it led to
PANTS: Mines metagenomes for uncharacterised PETase and MHETase candidates, ranked by catalytic triad and oxyanion hole
Playbook · 15 Sep 2026
Primary citation
Directed evolution of an efficient and thermostable PET depolymerase
Nat Catal 2022
Do it yourself
Field Guide: Xray and crystallisation
Unlocking Membrane Protein Structures: The Crystallization Revolution Reshaping Drug Discovery
Engineering Protein Stability: Unlocking the Secrets of Successful Crystallization
AXIS: Closing the Loop on Crystal Detection
Playbook
The Self-Driving Lab: How Automation and AI Are Redefining the Pace of Discovery
Tools
GOBSMACKED ↗
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TopPDBLX ↗
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BoltzMaker ↗
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