6C9U: Crystal structure of [KS3][AT3] didomain from module 3 of 6-deoxyerthronolide B synthase in complex with antibody fragment (Fab)
X-ray · 2.09 Å · released 23 May 2018 · Homo sapiens; Saccharopolyspora erythraea
How it was solved
- Method
- X-ray
- Resolution
- 2.09 Å
- Released
- 23 May 2018
- Rfree
- 0.210
- Rwork
- 0.166
- Clashscore
- 3.22
- Ramachandran outliers
- 0.00%
- Rotamer outliers
- 0.29%
Crystallisation
- Technique
- Vapor Diffusion, Sitting Drop
- Temperature
- 285.0 K
200 mM potassium citrate, 20%(w/v) PEG 3,350 and 12% ethylene glycol)
What it led to
No case written yet for this entry.
Primary citation
Structure–Function Analysis of the Extended Conformation of a Polyketide Synthase Module
Journal of the American Chemical Society 2018
Do it yourself
Field Guide: Xray and crystallisation
Unlocking Membrane Protein Structures: The Crystallization Revolution Reshaping Drug Discovery
Engineering Protein Stability: Unlocking the Secrets of Successful Crystallization
AXIS: Closing the Loop on Crystal Detection
Playbook
The Self-Driving Lab: How Automation and AI Are Redefining the Pace of Discovery
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