5T80: Crystal structure of 10E8 Fab in complex with the MPER epitope scaffold T117v2 and phosphatidic acid (06:0 PA)
X-ray · 2.62 Å · released 8 Mar 2017 · Homo sapiens; synthetic construct
Ligand: 44E
How it was solved
- Method
- X-ray
- Resolution
- 2.62 Å
- Released
- 8 Mar 2017
- Rfree
- 0.243
- Rwork
- 0.184
- Clashscore
- 6.58
- Ramachandran outliers
- 0.34%
- Rotamer outliers
- 5.47%
Crystallisation
- Technique
- Vapor Diffusion
- pH
- 9.5
- Temperature
- 295.15 K
50% PEG 400, 0.2M NaCl, 0.1 M CHES pH 9.5
What is new
No UniProt accession is mapped to this entry, so there is no target timeline.
What it led to
No case written yet for this entry.
Primary citation
Lipid interactions and angle of approach to the HIV-1 viral membrane of broadly neutralizing antibody 10E8: Insights for vaccine and therapeutic design
PLoS Pathogens 2017
Do it yourself
Field Guide: Xray and crystallisation
Unlocking Membrane Protein Structures: The Crystallization Revolution Reshaping Drug Discovery
Engineering Protein Stability: Unlocking the Secrets of Successful Crystallization
AXIS: Closing the Loop on Crystal Detection
Playbook
The Self-Driving Lab: How Automation and AI Are Redefining the Pace of Discovery
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