4RBO: Crystal structure of a Nanog homeobox (NANOG) from Homo sapiens at 3.30 A resolution
X-ray · 3.30 Å · released 1 Oct 2014 · Homo sapiens
How it was solved
- Method
- X-ray
- Resolution
- 3.30 Å
- Released
- 1 Oct 2014
- Rfree
- 0.268
- Rwork
- 0.235
- Clashscore
- 2.36
- Ramachandran outliers
- 0.00%
- Rotamer outliers
- 1.87%
Crystallisation
- Technique
- Vapor Diffusion, Sitting Drop
- pH
- 5.66
- Temperature
- 293.0 K
0.200M calcium acetate, 12.00% 2-propanol, 0.1M sodium cacodylate pH 5.66, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K
What it led to
No case written yet for this entry.
Primary citation
Structure-based discovery of NANOG variant with enhanced properties to promote self-renewal and reprogramming of pluripotent stem cells
Proceedings of the National Academy of Sciences of the United States of America 2015
Do it yourself
Field Guide: Xray and crystallisation
Unlocking Membrane Protein Structures: The Crystallization Revolution Reshaping Drug Discovery
Engineering Protein Stability: Unlocking the Secrets of Successful Crystallization
AXIS: Closing the Loop on Crystal Detection
Playbook
The Self-Driving Lab: How Automation and AI Are Redefining the Pace of Discovery
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