4D8D: Crystal structure of HIV-1 NEF Fyn-SH3 R96W variant
X-ray · 2.52 Å · released 16 Jan 2013 · Homo sapiens; Human immunodeficiency virus type 1 (BRU ISOLATE)
How it was solved
- Method
- X-ray
- Resolution
- 2.52 Å
- Released
- 16 Jan 2013
- Rfree
- 0.238
- Rwork
- 0.212
- Clashscore
- 4.52
- Ramachandran outliers
- 0.00%
- Rotamer outliers
- 4.17%
Crystallisation
- Technique
- Vapor Diffusion, Hanging Drop
- pH
- 8.4
- Temperature
- 293.0 K
0.3 M sodium potassium tartrate, 0.5 M bicine buffer , 1cmc D-octylglucopyranoside, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
What it led to
Compositional Protein Design: Building Proteins from 3D Ellipsoid Blueprints
Field Guide · 11 Aug 2025
Do it yourself
Field Guide: Xray and crystallisation
Unlocking Membrane Protein Structures: The Crystallization Revolution Reshaping Drug Discovery
Engineering Protein Stability: Unlocking the Secrets of Successful Crystallization
AXIS: Closing the Loop on Crystal Detection
Playbook
The Self-Driving Lab: How Automation and AI Are Redefining the Pace of Discovery
Tools
GOBSMACKED ↗
fold, dock, relax and annotate a protein-ligand complex on your own GPU, then find the crystal structure and g
CODSWALLOP ↗
every PDB entry for a protein family in one place: what was actually made, what it bound, what crystallised, a
TopPDBLX ↗
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BoltzMaker ↗
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