3HSA: Crystal structure of pleckstrin homology domain (YP_926556.1) from SHEWANELLA AMAZONENSIS SB2B at 1.99 A resolution
X-ray · 1.99 Å · released 23 Jun 2009 · Shewanella amazonensis SB2B
How it was solved
- Method
- X-ray
- Resolution
- 1.99 Å
- Released
- 23 Jun 2009
- Rfree
- 0.233
- Rwork
- 0.189
- Clashscore
- 6.55
- Ramachandran outliers
- 0.38%
- Rotamer outliers
- 1.62%
Crystallisation
- Technique
- Vapor Diffusion, Sitting Drop
- pH
- 7.5
- Temperature
- 293.0 K
5.0000% polyethylene glycol 3000, 22.0000% polyethylene glycol 400, 10.0000% Glycerol, 0.1M HEPES pH 7.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 293K, VAPOR DIFFUSION, SITTING DROP
What it led to
No case written yet for this entry.
Primary citation
Bacterial Pleckstrin Homology Domains: A Prokaryotic Origin for the PH Domain
Journal of Molecular Biology 2010
Do it yourself
Field Guide: Xray and crystallisation
Unlocking Membrane Protein Structures: The Crystallization Revolution Reshaping Drug Discovery
Engineering Protein Stability: Unlocking the Secrets of Successful Crystallization
AXIS: Closing the Loop on Crystal Detection
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The Self-Driving Lab: How Automation and AI Are Redefining the Pace of Discovery
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