3H41: CRYSTAL STRUCTURE OF A NLPC/P60 FAMILY PROTEIN (BCE_2878) FROM BACILLUS CEREUS ATCC 10987 AT 1.79 A RESOLUTION
X-ray · 1.79 Å · released 5 May 2009 · Bacillus cereus ATCC 10987
Ligands: DGL, ALA
How it was solved
- Method
- X-ray
- Resolution
- 1.79 Å
- Released
- 5 May 2009
- Rfree
- 0.197
- Rwork
- 0.163
- Clashscore
- 3.35
- Ramachandran outliers
- 0.33%
- Rotamer outliers
- 0.00%
Crystallisation
- Technique
- Vapor Diffusion, Sitting Drop
- pH
- 4.2
- Temperature
- 277.0 K
0.2000M NaCl, 50.0000% PEG-200, 0.1M Phosphate Citrate pH 4.2, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K
What it led to
No case written yet for this entry.
Primary citation
Structure of the γ-D-glutamyl-L-diamino acid endopeptidase YkfC from Bacillus cereus in complex with L-Ala-γ-D-Glu: insights into substrate recognition by NlpC/P60 cysteine peptidases
Acta Crystallographica Section F: Structural Biology and Crystallization Communications 2010
Do it yourself
Field Guide: Xray and crystallisation
Unlocking Membrane Protein Structures: The Crystallization Revolution Reshaping Drug Discovery
Engineering Protein Stability: Unlocking the Secrets of Successful Crystallization
AXIS: Closing the Loop on Crystal Detection
Playbook
The Self-Driving Lab: How Automation and AI Are Redefining the Pace of Discovery
Tools
GOBSMACKED ↗
fold, dock, relax and annotate a protein-ligand complex on your own GPU, then find the crystal structure and g
CODSWALLOP ↗
every PDB entry for a protein family in one place: what was actually made, what it bound, what crystallised, a
TopPDBLX ↗
every crystallisation condition in the protein data bank, parsed and indexed: what worked, how often, and how
FlexAppeal ↗
Set up protein molecular dynamics without writing the script: every OpenMM option explained, a self-contained
BoltzMaker ↗
Build and analyze Boltz-2 protein structure prediction campaigns: a guided input wizard, YAML generation, and