Field Guide
Cryo-EM
Single particles, tomography and membrane proteins: where cryo-EM now beats crystallography and where it still does not.
Other chapters
X-ray and crystallisation
Cryo-EM
Fundamentals
Prediction and validation
Protein design
AI workflows and chemistry tools
Docking, screening and simulation
Try it
FAFFAbout ↗
forecasts where a protein is likely to stall on the road to a structure, from 335,771 real attempts in the Protein Structure Initiative arch…
ALPHABETTI ↗
a protein sequence logo in 3D, wrapped around its own predicted structure. The amino acid letters themselves, stacked by how strongly a lang…
ButtFold ↗
a protein folds in your browser and the trajectory becomes music: contacts forming are note onsets, hydropathy sets pitch, secondary structu…
TopPDBLX ↗
every crystallisation condition in the protein data bank, parsed and indexed: what worked, how often, and how little of it is specific to yo…
AlphaFraud ↗
Catch AlphaFold where the fold is wrong: weekly, automatically, on freshly deposited human structures
ChatPDB ↗
A protein-structure-literate AI assistant for analyzing proteins: RAG for facts, a QLoRA-tuned model for behaviour, and live Biopython/gemmi…
6 articles
Outrunning Diffusion: The Fast Lane in CryoEM Grid Preparation
Outrunning Diffusion: The Fast Lane in CryoEM Grid Preparation Outrunning protein diffusion to the air–water interface in cryoEM Hyperquenching: Nanosecond vitrification …
Revolutionary Cryo-EM Breakthrough: Di-Gembodies Transform Small Protein Structure Determination
Revolutionary Cryo-EM Breakthrough: Di-Gembodies Transform Small Protein Structure Determination Di-Gembodies Size Barrier Elimination: Di-Gembodies enable cryo-EM struct…
Revolutionizing Protein Structure Determination: How MICA Combines Cryo-EM and AlphaFold3 for Unprecedented Accuracy
Revolutionizing Protein Structure Determination: How MICA Combines Cryo-EM and AlphaFold3 for Unprecedented Accuracy Revolutionary Integration: MICA combines cryo-EM expe…
Revolutionizing Protein Structure Analysis: Cryo-EM without Purification Accelerates the Future of Structural Biology
Revolutionizing Protein Structure Analysis: Cryo-EM without Purification Accelerates the Future of Structural Biology New workflow enables solving protein structures via …
Cryo-EM and the New Frontier in Membrane Protein Drug Design
Cryo-EM and the New Frontier in Membrane Protein Drug Design Cryo-EM advancements now enable the high-resolution structural determination of challenging membrane proteins…
Beyond the Microscope: How Cryo-ET is Revealing Disease in Its Native State
Beyond the Microscope: How Cryo-ET is Revealing Disease in Its Native State Cryo-electron tomography (Cryo-ET) allows scientists to visualize protein structures within th…